Human IL-10 ELISA Kit (Interleukin-10)

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.4 pg/ml
  • Range: 9.4 pg/ml – 3000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human IL-10 ELISA Kit (Interleukin-10)

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
supernatant5  5.2%
Inter-assay
SamplenMeanSDCV%
supernatant3  3.2%

Sample type

Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

1.4 pg/ml

Range

9.4 pg/ml – 3000 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant108105% – 111%
Serum109108% – 112%
Cell culture media106102% – 111%
Hep Plasma116113% – 117%
EDTA Plasma109103% – 118%
Cit plasma111105% – 119%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human
Does not react with: Cow

Product overview

Human IL-10 ELISA Kit (Interleukin-10) (ab185986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 (Interleukin-10) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-10 (Interleukin-10) with 1.4 pg/ml sensitivity.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human IL-10 Capture Antibody1 x 600µl
10X Human IL-10 Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml
Antibody Diluent CPI – HAMA Blocker (ab193969)1 x 6ml
Human IL-10 Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NBP1 x 20ml
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Immunology
  •  
  • Adaptive Immunity
  •  
  • T Cells
  •  
  • Non-CD
  • Immunology
  •  
  • Innate Immunity
  •  
  • Cytokines
  •  
  • Interleukins
  • Cancer
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  • Tumor immunology
  •  
  • Cytokines
  •  
  • Interleukins
  • Immunology
  •  
  • Adaptive Immunity
  •  
  • Regulatory T Cells
  • Kits/ Lysates/ Other
  •  
  • Kits
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  • ELISA Kits
  •  
  • ELISA Kits
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  • Cytokines and cytokine receptors ELISA kits
  • Neuroscience
  •  
  • Processes

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Tissue specificity

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Sequence similarities

Belongs to the IL-10 family.

Cellular localization

Secreted.

Protocols