Mouse LBP ELISA Kit (LPS Binding Protein)

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.34 pg/ml
  • Range: 50 pg/ml – 3200 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

Product name

Mouse LBP ELISA Kit (LPS Binding Protein)

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Serum8  7.5%
Inter-assay
SamplenMeanSDCV%
Serum3  10.1%

Sample type

Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

8.34 pg/ml

Range

50 pg/ml – 3200 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Serum8483% – 85%
Cell culture media9992% – 103%
Hep Plasma10095% – 104%
EDTA Plasma9796% – 99%
Cit plasma103101% – 105%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Mouse

Product overview

Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LBP (LPS Binding Protein) protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LBP (LPS Binding Protein) with 8.34 pg/ml sensitivity.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Mouse LBP (LPS Binding Protein) Capture Antibody1 x 600µl
10X Mouse LBP (LPS Binding Protein) Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
Antibody Diluent CPR1 x 6ml
Mouse LBP (LPS Binding Protein) Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Immunology
  •  
  • Innate Immunity
  •  
  • Macrophage / Inflamm.
  • Microbiology
  •  
  • Protein
  •  
  • Human Protein
  •  
  • Defensin
  • Immunology
  •  
  • Innate Immunity
  •  
  • TLR Signaling

Function

Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor.

Sequence similarities

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Cellular localization

Secreted.

Protocols