Human BDNF ELISA Kit

SKU: N/A Categories: ,

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.4 pg/ml
  • Range: 15.6 pg/ml – 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human BDNF ELISA Kit
See all BDNF kits

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Serum5  2.8%
Inter-assay
SamplenMeanSDCV%
Serum3  5.3%

Sample type

Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

2.4 pg/ml

Range

15.6 pg/ml – 1000 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant106103% – 109%
Serum9794% – 104%
Hep Plasma10396% – 112%
EDTA Plasma9885% – 108%
Cit plasma10395% – 111%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human

Product overview

Human BDNF ELISA Kit (ab212166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BDNF protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BDNF with 2.4 pg/ml sensitivity.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


SPECIES REACTIVITY


This kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein. 


ASSAY SPECIFICITY


This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only.


Cell and tissue extract samples have not been tested with this kit.


CROSS REACTIVITY


Recombinant TRKB was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity.  No cross-reactivity was observed.


INTERFERENCE


Recombinant TRKB was prepared at 50 ng/mL and 1 ng/mL and tested for interference with human BDNF at 200 pg/mL. 39% Inference was observed with 1ng/mL and 76% Interference was observed with 50 ng/mL.

Notes

BDNF (Brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human BDNF Capture Antibody1 x 600µl
10X Human BDNF Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
Antibody Diluent CPI – HAMA Blocker (ab193969)1 x 6ml
Human BDNF Lyophilized Recombinant Protein2 vials
Plate Seals1 unit
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Neuroscience
  •  
  • Neurology process
  •  
  • Growth and Development
  •  
  • Neurotrophins
  • Neuroscience
  •  
  • Neurology process
  •  
  • Neurodegenerative disease
  •  
  • Huntington’s disease
  • Neuroscience
  •  
  • Neurology process
  •  
  • Neurodegenerative disease
  •  
  • Alzheimer’s disease
  •  
  • Other
  • Cardiovascular
  •  
  • Atherosclerosis
  •  
  • Diabetes associated
  • Cardiovascular
  •  
  • Atherosclerosis
  •  
  • Thrombosis
  •  
  • Other
  • Metabolism
  •  
  • Types of disease
  •  
  • Diabetes
  • Metabolism
  •  
  • Types of disease
  •  
  • Obesity
  • Neuroscience
  •  
  • Development
  • Neuroscience
  •  
  • Processes

Function

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Tissue specificity

Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Involvement in disease

Bulimia nervosa 2
Congenital central hypoventilation syndrome

Sequence similarities

Belongs to the NGF-beta family.

Post-translational
modifications

The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).

Cellular localization

Secreted.

Protocols