Native Human IgE protein (Azide free)

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Key features and details

  • Expression system: Native
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 25.000 Eu/mg
  • Active: Yes
  • Suitable for: SDS-PAGE, IHC – Wholemount, WB, ELISA, ICC

Overview

Product name

Native Human IgE protein (Azide free)

Biological activity

1 mg of ab65866 is 380,000± 50,000 IU/m.

Purity

> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.

Endotoxin level

< 25.000 Eu/mg

Expression system

Native

Accession

Protein length

Full length protein

Animal free

No

Nature

Native

Amino Acid Sequence 1

Species

Human

Additional sequence information

Observed band of ~185kDa on SDS-PAGE

Description

Native human IgE protein (Azide free)

Properties

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

IHC – Wholemount

Western blot

ELISA

Immunocytochemistry

Form

Liquid

Additional notes

This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

– Low batch-to-batch variation

– Higher degree of purity (>90%)

– A more uniform product (monoclonal and not polyclonal)

Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.

Concentration

  • 100 µg at 1 mg/ml
  • 1 mg at 1 mg/ml

Protocols