Pig IL-1 beta ELISA Kit

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Key features and details

  • Sensitivity: 6 pg/ml
  • Range: 8.23 pg/ml – 6000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Pig

Overview

Product name

Pig IL-1 beta ELISA Kit
See all IL-1 beta kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/ml

Range

8.23 pg/ml – 6000 pg/ml

Recovery

90 %

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant96.9189% – 104%
Serum97.3288% – 105%
Plasma87.7580% – 92%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Pig

Product overview

Abcam’s IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.


 


This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
20X Wash Buffer1 x 25ml
5X Assay Diluent B1 x 15ml
600X HRP-Streptavidin Concentrate1 x 200µl
Assay Diluent C1 x 30ml
Biotinylated anti-Pig IL-1 beta2 vials
IL1 beta Microplate (12 x 8 wells)1 unit
Recombinant Pig IL-1 beta Standard (lyophilized)2 vials
Stop Solution1 x 8ml
TMB One-Step Substrate Reagent1 x 12ml

Research areas

  • Immunology
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Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Tissue specificity

Expressed in activated monocytes/macrophages (at protein level).

Sequence similarities

Belongs to the IL-1 family.

Post-translational
modifications

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Cellular localization

Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.

Protocols