Rat IL-1 beta ELISA Kit

SKU: N/A Categories: ,

Key features and details

  • Sensitivity: 80 pg/ml
  • Range: 68.59 pg/ml – 50000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

Product name

Rat IL-1 beta ELISA Kit
See all IL-1 beta kits

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Overall   < 10%
Inter-assay
SamplenMeanSDCV%
Overall   < 12%

Sample type

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Sensitivity

< 80 pg/ml

Range

68.59 pg/ml – 50000 pg/ml

Recovery

116 %

Sample specific recovery
Sample typeAverage %Range
Cell culture extracts96.2288% – 105%
Tissue Extracts136.5126% – 145%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Rat

Product overview

Abcam’s IL-1 beta Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.


This assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

Notes

 

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
200X HRP-Streptavidin Concentrate1 x 200µl
20X Wash Buffer1 x 25ml
2X Cell Lysis Buffer1 x 5ml
5X Assay Diluent1 x 15ml
5X Sample Diluent Buffer1 x 10ml
Biotinylated anti-rat IL-1 beta2 vials
IL-1 beta Microplate (12 x 8 wells)1 unit
Recombinant rat IL-1 beta Standard (lyophilized) 2 vials
Stop Solution1 x 8ml
TMB One-Step Substrate Reagent1 x 12ml

Research areas

  • Immunology
  •  
  • Innate Immunity
  •  
  • Cytokines
  •  
  • Interleukins
  • Microbiology
  •  
  • Organism
  •  
  • Virus
  •  
  • RNA Virus
  •  
  • ssRNA positive strand virus
  •  
  • SARS Coronavirus
  • Cancer
  •  
  • Tumor immunology
  •  
  • Cytokines
  •  
  • Interleukins
  • Cardiovascular
  •  
  • Atherosclerosis
  •  
  • Vascular Inflammation
  •  
  • Inflammatory mediators
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • ELISA Kits
  •  
  • ELISA Kits
  •  
  • Cytokines and cytokine receptors ELISA kits
  • Metabolism
  •  
  • Types of disease
  •  
  • Obesity
  • Neuroscience
  •  
  • Processes

Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Tissue specificity

Expressed in activated monocytes/macrophages (at protein level).

Sequence similarities

Belongs to the IL-1 family.

Post-translational
modifications

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Protocols