Glutathione Peroxidase Assay Kit (Colorimetric)

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Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 40 min
  • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Platelets, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.5 mU/ml

Overview

Product name

Glutathione Peroxidase Assay Kit (Colorimetric)
See all Glutathione Peroxidase kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Urine, Serum, Plasma, Platelets, Other biological fluids, Tissue Extracts

Assay type

Enzyme activity

Sensitivity

0.5 mU/ml

Assay time

0h 40m

Species reactivity

Reacts with: Mammals, Other species

Product overview

Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

 

In the glutathione peroxidase assay protocol, glutathione peroxidase (GPx) oxidizes GSH to produce GSSG as part of the reaction in which it reduces cumene hydroperoxide. Glutathione reductase (GR) then reduces the GSSG to produce GSH, and in the same reaction consumes NADPH. The decrease of NADPH (measured at OD=340 nm) is proportional to GPx activity. 

 

The assay has a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.

Glutathione peroxidase assay protocol summary:
– add samples and standards to wells
– add reaction mix and incubate for 15 min at room temp to deplete all GSSG in sample
– add cumene hydroperoxide
– analyze with microplate reader immediately, and after at least 5 min

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K762 Glutathione Peroxidase Activity Colorimetric Assay Kit. K762-100 is the same size as the 100 test size of ab102530.

The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. 

Platform

Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
ComponentsIdentifier100 tests2000 tests
Cumene HydroperoxideYellow1 vial20 vials
GlutathioneBrown1 vial20 vials
Glutathione ReductaseGreen1 vial20 vials
GPx Assay BufferWM1 x 50ml20 x 50ml
GPx Positive ControlRed1 vial20 vials
NADPHBlue1 vial20 vials

Research areas

  • Signal Transduction
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  • Metabolism
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  • Mitochondrial
  • Cell Biology
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  • Other Antibodies
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  • Oxidative Stress
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • Cell Damage Kits
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  • Oxidative stress
  • Kits/ Lysates/ Other
  •  
  • Kits
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  • Cell Metabolism Kits
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  • Coenzyme and cofactor assay kits
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  • Glutathione assay kits
  • Metabolism
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  • Pathways and Processes
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  • Mitochondrial Metabolism
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  • Mitochondrial markers
  • Metabolism
  •  
  • Pathways and Processes
  •  
  • Redox metabolism
  •  
  • Antioxidants
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • Cell Damage Kits
  •  
  • Antioxidant

Relevance

Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.

Alternative names

  • GPX

Protocols