Anti-Collagen I antibody

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Key features and details

  • Rabbit polyclonal to Collagen I
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

Product name

Anti-Collagen I antibody

Description

Rabbit polyclonal to Collagen I

Host species

Rabbit

Specificity

This product is not recommended for use under denaturing conditions in WB. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins here.

ab34710 has <5% cross-reactivity with Collagen III

Customers have been successful using ab34710 in this application, please see references below (Tillgren V  et al. Biol Chem 290:918-25; 2015).

Positive Control: Human stomach, skin and adrenal gland tissue lysates.

Tested applications

Suitable for: WBmore details

Species reactivity

Reacts with: Human

Immunogen

Full length native protein (purified) corresponding to Human Collagen I aa 1-1464. Collagen Type I from human and bovine placenta.
Database link: P02452

Positive control

WB: human collagen.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.8766% Sodium chloride, 0.42% Potassium phosphate

Concentration

Batch dependent within range: 100 µg at 1 – 1.2 mg/ml

Purity

Immunogen affinity purified

Purification notes

ab34710 has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered.

Clonality

Polyclonal

Isotype

IgG

Research areas

  • Signal Transduction
  •  
  • Cytoskeleton / ECM
  •  
  • Extracellular Matrix
  •  
  • ECM Proteins
  •  
  • Collagen
  • Stem Cells
  •  
  • Mesenchymal Stem Cells
  •  
  • Osteogenesis

Protocols