Pig IL-6 ELISA Kit

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Key features and details

  • Sensitivity: 45 pg/ml
  • Range: 40.96 pg/ml – 10000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Pig

Overview

Product name

Pig IL-6 ELISA Kit

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 45 pg/ml

Range

40.96 pg/ml – 10000 pg/ml

Recovery

89 %

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant108.395% – 117%
Serum93.4785% – 102%
Plasma85.7367% – 99%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Pig

Product overview

Abcam’s IL-6 (Interleukin-6) Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-6 in serum, plasma and cell culture supernatants. 


This assay employs an antibody specific for pig IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
 

Research areas

  • Immunology
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  • Innate Immunity
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  • Macrophage / Inflamm.
  • Immunology
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  • Innate Immunity
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  • Cytokines
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  • Interleukins
  • Neuroscience
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  • Neurology process
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  • Metabolism
  • Microbiology
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  • Organism
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  • Virus
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  • RNA Virus
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  • ssRNA positive strand virus
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  • SARS Coronavirus
  • Cancer
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  • Invasion/microenvironment
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  • Angiogenesis
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  • Angiogenic growth factors
  • Cancer
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  • Tumor immunology
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  • Cytokines
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  • Interleukins
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • ELISA Kits
  •  
  • ELISA Kits
  •  
  • Cytokines and cytokine receptors ELISA kits
  • Metabolism
  •  
  • Types of disease
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  • Obesity
  • Metabolism
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  • Types of disease
  •  
  • Metabolic disorders
  • Neuroscience
  •  
  • Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Protocols