Human IL-6 ELISA Kit

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.6 pg/ml
  • Range: 7.8 pg/ml – 500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human IL-6 ELISA Kit
See all IL-6 kits

Detection method

Colorimetric

Precision

Intra-assay
SamplenMeanSDCV%
Cell Extract5  2.1%
Inter-assay
SamplenMeanSDCV%
Cell Extract3  2.4%

Sample type

Cell culture supernatant, Serum, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

1.6 pg/ml

Range

7.8 pg/ml – 500 pg/ml

Recovery

 

Sample specific recovery
Sample typeAverage %Range
Serum8277% – 84%
Cell culture media10097% – 103%
EDTA Plasma8280% – 85%
Cit plasma8481% – 85%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human

Product overview

Human IL-6 ELISA kit (ab178013) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-6 with 1.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        -Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


ASSAY SPECIFICITY This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.


CROSS REACTIVITY Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.


INTERFERENCE Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed. 18.Species Reactivity This kit recognizes Human IL-6 protein. Recombinant mouse IL-6 was prepared at 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Please contact our Technical Support team for more information

Notes

Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Platform

Microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.
Components1 x 96 tests
10X Human IL-6 Detector Antibody1 x 600µl
10X Wash Buffer PT (ab206977)1 x 20ml
Antibody Diluent 5BI1 x 6ml
Human IL-6 Capture Antibody (Lyophilized)1 vial
Human IL-6 Lyophilized Recombinant Protein (ab9627)2 vials
Plate Seals1 unit
Sample Diluent NS (ab193972)1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
Stop Solution1 x 12ml
TMB Development Solution1 x 12ml

Research areas

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.
  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins
  • Neuroscience
  • Neurology process
  • Metabolism
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • ssRNA positive strand virus
  • SARS Coronavirus
  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Angiogenic growth factors
  • Cancer
  • Tumor immunology
  • Cytokines
  • Interleukins
  • Kits/ Lysates/ Other
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  • ELISA Kits
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  • Interleukin ELISA kits
  • Kits/ Lysates/ Other
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  • Cytokines and cytokine receptors ELISA kits
  • Metabolism
  • Types of disease
  • Obesity
  • Metabolism
  • Types of disease
  • Metabolic disorders
  • Neuroscience
  • Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Involvement in disease

Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational
modifications

N- and O-glycosylated.

Cellular localization

Secreted.

Protocols