Human Insulin ELISA Kit

SKU: N/A Categories: ,

Key features and details

  • Sensitivity: 4 µlU/ml
  • Range: 4.69 µlU/ml – 300 µlU/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

Product name

Human Insulin ELISA Kit
See all Insulin kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 4 µlU/ml

Range

4.69 µlU/ml – 300 µlU/ml

Recovery

89 %

Sample specific recovery
Sample typeAverage %Range
Cell culture supernatant76.1668% – 88%
Serum91.483% – 102%
Plasma99.0373% – 128%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig

Product overview

Abcam’s Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants.


This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


This kit detects both insulin and proinsulin.


Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (ab200011) from our SimpleStep ELISA® range.

Notes

Optimization may be required with urine samples.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.
Components1 x 96 tests
20X Wash Buffer1 x 25ml
500X HRP-Streptavidin Concentrate1 x 200µl
5X Assay Diluent B1 x 15ml
Assay Diluent A1 x 30ml
Biotinylated anti-Human Insulin2 vials
Insulin Microplate (12 strips x 8 wells)1 unit
Recombinant Human Insulin Standard (lyophilized)2 vials
Stop Solution1 x 8ml
TMB One-Step Substrate Reagent1 x 12ml

Research areas

  • Signal Transduction
  •  
  • Growth Factors/Hormones
  •  
  • Insulin / Insulin-like
  • Neuroscience
  •  
  • Neurology process
  •  
  • Metabolism
  • Signal Transduction
  •  
  • Metabolism
  •  
  • Lipid metabolism
  • Cancer
  •  
  • Growth factors
  •  
  • Insulin and insulin-like
  • Cardiovascular
  •  
  • Atherosclerosis
  •  
  • Diabetes associated
  • Kits/ Lysates/ Other
  •  
  • Kits
  •  
  • ELISA Kits
  •  
  • ELISA Kits
  •  
  • Growth factors and hormones ELISA kits
  • Metabolism
  •  
  • Types of disease
  •  
  • Diabetes
  • Metabolism
  •  
  • Types of disease
  •  
  • Obesity
  • Metabolism
  •  
  • Types of disease
  •  
  • Heart disease

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Involvement in disease

Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

Sequence similarities

Belongs to the insulin family.

Cellular localization

Secreted.

Protocols