Anti-beta Actin antibody [mAbcam 8226] – Loading Control

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Key features and details

  • Mouse monoclonal [mAbcam 8226] to beta Actin – Loading Control
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

Product name

Anti-beta Actin antibody [mAbcam 8226] – Loading Control
See all beta Actin primary antibodies

Description

Mouse monoclonal [mAbcam 8226] to beta Actin – Loading Control

Host species

Mouse

Specificity

Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.

Tested applications

Suitable for: ICC/IFIHC-PWBmore details

Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey, Zebrafish, African green monkey, Chinese hamster, Armenian hamster

Immunogen

Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
Database link: P60709
(Peptide available as ab13772)

Positive control

  • WB, ICC/IF: HeLa, Jurkat, A431, HEK293, NIH 3T3, PC12 cells. IHC-P: Human colon (FFPE), Rat colon (FFPE) tissue. Hela cells

General notes

Western blot protocol advice:

We recommend blocking with 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please see the comparison data in the images section. If milk block is required, we recommend using ab8224 mouse monoclonal [mAbcam 8224] to beta actin. Contact our Scientific Support team for more information or advice.

This antibody clone [mAbcam 8226] is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact [email protected] or you can find further information here.

 

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Concentration

  • 100 µg at 1 mg/ml

Purity

Protein G purified

Clonality

Monoclonal

Clone number

mAbcam 8226

Myeloma

Sp2/0-Ag14

Isotype

IgG1

Light chain type

kappa

Research areas

  • Isotype/Loading Controls
  •  
  • Loading Controls
  •  
  • Beta Actin
  • Tags & Cell Markers
  •  
  • Subcellular Markers
  •  
  • Cytoskeleton
  •  
  • Actin
  • Signal Transduction
  •  
  • Cytoskeleton / ECM
  •  
  • Cytoskeleton
  •  
  • Microfilaments
  •  
  • Actin etc
  •  
  • Actin

Protocols